Journal: Communications Medicine
Article Title: Spatial omics-based machine learning algorithms for the early detection of hepatocellular carcinoma
doi: 10.1038/s43856-024-00677-7
Figure Lengend Snippet: a Slides are spotted with 16 antibodies (top to bottom in columns) to the following antibodies: (1) anti-Alpha 1 Antitrypsin, (2) anti- Alpha 1B-Glycopotein, (3) anti-alpha 1-Acid Glycoprotein, (4) anti-Alpha-2-Macroglobulin, (5) anti-Angiotensinogen II/III, (6) anti-Apolipoprotein D, (7) anti-Apolipoprotein H (ApoH), (8) anti-Ceruloplasmin, (9) anti-Clusterin, 10) anti-Fetuin, 11) anti-Haptoglobin, 12) anti-Hemopexin, (13) anti histidine-proline rich glycoprotein; (14) anti-IgG, (15) anti-Transferrin; (16) anti-Vitamin D Binding Protein. B-D) Example of GlycoTyper data for the 16 captured proteins for N-glycan at 2539.881 ( b , e ), N-glycan at 2174.654 ( c , f ) and N-glycan at 1809.639 ( d , g ). b – d are from patients with HCC, while e – g are from patient with cirrhosis. h Bar graph with individual data points showing the mean (with SD) level of N-glycan at 2539.907 on haptoglobin; i Bar graph with individual data points showing the mean (with SD) level of N-glycan at 1663.581 on transferrin. j , k Similar analysis on angiotensinogen ( j ) hemopexin ( k ). l ROC curves for Model G, AFP and AFP-L3 of discriminant ability to classify all or early-stage HCC from cirrhosis. Where statistical difference exists, the p value is provided and error bars indicate the standard deviation.
Article Snippet: Goat anti-Human IgG (#A80-104A) and Goat anti-Human Transferrin (#A80-128A) antibodies were obtained from Bethyl Laboratories (Montgomery, TX).
Techniques: Binding Assay, Glycoproteomics, Standard Deviation
Bethyl is a verified supplier 
